Goals: This examine aimed to judge the truthfulness of sufferers about their pre-appointment COVID-19 screening assessments at a dental clinic.
Strategies: A complete of 613 sufferers have been recruited for the examine from the dental clinic on the College of Dentistry, Najran College, Saudi Arabia. The information assortment was performed in three components from the sufferers who visited the hospital to obtain dental therapy. The primary half included the socio-demographic traits of the sufferers and the COVID-19 swab assessments carried out throughout the previous 14 days. The second half was the medical examination, and the third half was a affirmation of the swab check taken by the affected person by checking the Hesen web site utilizing the affected person ID. After knowledge assortment, statistical evaluation was carried out utilizing SPSS 26.0. Descriptive evaluation was performed and expressed as imply, normal deviation, frequency, and share (%). A cross-tabulation, additionally described as a contingency desk, was used to determine tendencies and patterns throughout knowledge and clarify the correlation between completely different variables.
Outcomes: It was seen from the standing of the swab check inside 14 days of the affected person’s arrival on the hospital for the dental therapy that 18 (2.9%) sufferers lied in regards to the pre-treatment swab check inside 14 days, and 595 (97.1%) have been truthful. The noticed and anticipated counts confirmed throughout genders and analysis a statistically important distinction (p < 0.001), and there was no important distinction seen throughout completely different age teams (p = 0.064) of the sufferers.
Conclusions: Dental healthcare employees are nervous and assume a excessive danger of COVID-19 an infection because the sufferers are usually not truthful in regards to the pre-treatment COVID-19 swab check. Routine speedy assessments on sufferers and the healthcare workers are a possible possibility for reducing general dangers.
milansystemmilansystem
cDNA cloning, expression, and antifungal exercise of chitinase from Ficus microcarpa latex: distinction in antifungal motion of chitinase with and with out chitin-binding area
A chitin-binding area might contribute to the antifungal means of chitinase via its affinity to the fungal lateral wall by hydrophobic interactions. Complementary DNA encoding the antifungal chitinase of gazyumaru (Ficus microcarpa), designated GlxChiB, was cloned and expressed in Escherichia coli cells. The outcomes of cDNA cloning confirmed that the precursor of GlxChiB has an N-terminal endoplasmic reticulum focusing on sign and C-terminal vacuolar focusing on sign, whereas mature GlxChiB consists of an N-terminal carbohydrate-binding module family-18 area (CBM18) and a C-terminal glycoside hydrolase family-19 area (GH19) with a brief linker. To make clear the function of the CBM18 area within the antifungal exercise of chitinase, the recombinant GlxChiB (wild sort) and its catalytic area (CatD) have been used in quantitative antifungal assays below completely different ionic strengths and microscopic observations towards the fungus Trichoderma viride.
The antifungal exercise of the wild sort was stronger than that of CatD below all ionic energy circumstances used on this assay; nevertheless, the antifungal exercise of CatD grew to become weaker with growing ionic energy, whereas that of the wild sort was maintained. The outcomes at excessive ionic energy additional verified the contribution of the CBM18 area to the antifungal means of GlxChiB. The microscopic observations clearly confirmed that the wild sort acted on each the ideas and the lateral wall of fungal hyphae, whereas CatD acted solely on the ideas. These outcomes recommend that the CBM18 area might contribute to the antifungal means of chitinase via its affinity to the fungal lateral wall by hydrophobic interactions
Utility of the modified handmade cloning method to pigs
Though somatic cell nuclear switch (SCNT) is often employed to provide cloned animals in laboratories, this system is pricey and inefficient. Subsequently, the handmade cloning (HMC) method has been urged to simplify and advance the cloning course of, nevertheless, HMC wastes many oocytes and results in mitochondrial heteroplasmy. To resolve these issues, we suggest a modified handmade cloning (mHMC) method that makes use of easy laboratory tools, i.e., a Pasteur pipette and an alcohol lamp, making use of it to porcine embryo cloning. To validate the utility of mHMC to pig cloning, embryos produced via SCNT and mHMC are in contrast utilizing a number of strategies, equivalent to enucleation effectivity, oxidative stress, embryo developmental competence, and gene expression.
The outcomes present no important variations between strategies besides within the enucleation effectivity. The 8-cell and 16-cell embryo developmental competence and Oct4 expression ranges exhibit important variations. Nevertheless, the blastocyst charge just isn’t considerably completely different between mHMC and SCNT. This examine verifies that cloned embryos derived from the 2 strategies exhibit related technology and developmental competence. Thus, we propose that mHMC might exchange SCNT for easier and cheaper porcine cloning.
Molecular cloning and characterization of high-affinity potassium transporter (AlHKT2;1) gene promoter from halophyte Aeluropus lagopoides
HKT subfamily II capabilities as Na+– Ok+ co-transporter and prevents crops from salinity stress. A 760 bp promoter area of AlHKT2;1 was remoted, sequenced and cloned. The total size promoter D1, has many cis-regulatory parts like MYB, MBS, W field, ABRE and many others. concerned in abiotic stress responses. D1 and subsequent 5′ deletions have been cloned into pCAMBIA1301 and studied for its efficacy in stress circumstances in heterologous system. Blue color staining was noticed in flower petals, anther lobe, and dehiscence slit of anther in T0 crops. The T1 seedling confirmed staining in leaf veins, shoot vasculature and root besides root tip. T1 seedlings have been subjected to NaCl, KCl and NaCl + KCl and ABA stresses. GUS exercise was quantified by 4-methylumbelliferyl glucuronide (4-MUG) assay below management and stress circumstances.
The smallest deletion- D4 additionally confirmed GUS expression however highest exercise was noticed in D2 as in comparison with full size promoter and different deletions. The electrophoretic mobility shift assay utilizing stress-induced protein with completely different promoter deletions revealed extra outstanding binding in D2. These outcomes recommend that AlHKT2;1 promoter is concerned in abiotic stress response and deletion D2 is perhaps ample to drive the stress-inducible expression of assorted genes concerned in offering stress tolerance in crops
Stem cell therapies and benefaction of somatic cell nuclear switch cloning in COVID-19 period
Background: The worldwide well being emergency of COVID-19 has necessitated the event of a number of therapeutic modalities together with vaccinations, antivirals, anti-inflammatory, and cytoimmunotherapies, and many others. COVID-19 sufferers endure from harm to varied organs and vascular buildings, so that they current a number of well being crises. Mesenchymal stem cells (MSCs) are of curiosity to deal with acute respiratory misery syndrome (ARDS) attributable to SARS-CoV-2 an infection.
Important physique: Stem cell-based therapies have been verified for potential advantages in copious preclinical and medical research. MSCs confer potential advantages to develop numerous cell varieties and organoids for finding out virus-human interplay, drug testing, regenerative drugs, and immunomodulatory results in COVID-19 sufferers. Aside from paving the methods to reinforce stem cell analysis and therapies, somatic cell nuclear switch (SCNT) holds distinctive means for a wide selection of well being purposes equivalent to patient-specific or isogenic cells for regenerative drugs and breeding transgenic animals for biomedical purposes. Being a potent cell genome-reprogramming instrument, the SCNT has elevated prominence of recombinant therapeutics and mobile drugs within the present period of COVID-19. As SCNT is used to generate patient-specific stem cells, it avoids dependence on embryos to acquire stem cells.
Conclusions: The nuclear switch cloning, being a really perfect instrument to generate cloned embryos, and the embryonic stem cells will increase drug testing and mobile drugs in COVID-19.
Molecular cloning of duck CD40 and its immune operate analysis
Cosignal molecules are cell floor molecules that transduce alerts to different cells to modulate immune response positively (costimulate) or negatively (cosuppress). Costimulatory alerts are key elements in figuring out whether or not T/B cells are able to responding to particular antigens and in the end mediating an applicable immune response. On this examine, the cDNA sequence containing the whole coding body of the costimulatory molecule duck CD40 gene was cloned and reported for the primary time, and its mediated antiviral innate immune was verified in vitro. Outcomes urged duck CD40 molecule performs an vital function within the innate immune responsiveness towards some viruses. These knowledge will likely be useful for the additional perceive of the avian immune system.
Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Utility within the Deconstruction of Corn Stover
Xylanase performs an important function within the environment friendly utilization of xylan, which accounts for as much as 30% of plant dry matter. Nevertheless, the manufacturing value of xylanase stays excessive, and the enzymatic traits of xylanases of most microorganisms are usually not appropriate for industrial manufacturing. Subsequently, it’s of nice significance to find and develop new and environment friendly xylanases. On this examine, the xylanase gene TAX1 (672 bp cDNA) was cloned from Trichoderma atroviride 3.3013 and expressed in Pichia pastoris. The TAX1 gene encoded a 223-amino acid protein (TAX1) with a molecular weight of 24.2 kDa which confirmed excessive similarity to glycoside hydrolase household 11. Enzyme exercise assay verified that the recombinant xylanase TAX1 had optimum exercise (215.Three IU/mL) at 50°C and pH 6.0.
Steady working circumstances have been measured as pH 4.0-7.Zero and 40-60°C. By including Zn2+, the relative enzymatic exercise of recombinant TAX1 was enhanced by 26%. The recombinant xylanase confirmed excessive exercise towards birchwood xylan and corn stover. The Okm and Okcat for xylan and corn stover have been 0.36 mg/mL and 0.204 S-1 and 0.48 mg/mL and 0.149 S-1, respectively. The enzymatic exercise of the TAX1 produced by P. pastoris was about 2.4-Four occasions increased that straight remoted from T. atroviride, so engineered P. pastoris for xylanase manufacturing may very well be a really perfect candidate for industrial enzyme manufacturing.
Description: Human KIR2DL3,also known as Killer cell immunoglobulin-like receptor 2DL3, KIR2DS5 and KIRCL23. GenBank Accession No. NM_015868 a. a. 22-245, fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-Tag™ and expressed in a HEK293 cell expression system. MW=53 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human CD48, Fc fusion protein, also known as Cluster of Differentiation 48, BLAST-1, or SLAMF2. GenBank Accession No. NM_001778, a.a. 27-220 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 using Avi-tag™ technology. MW = 51 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human PDPN, known as Podoplanin, GenBank Accession No. NM_006474, a.a. 23-131, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 40 kDa.
Description: Human CD22 also known as cluster of differentiation-22, and SIGLEC-2, GenBank Accession No. NM_001771, a.a. 100-330 fused to Fc region of Human IgG1 with C-terminal Avi-Tag™. and expressed in a HEK293 cell expression system. MW = 104 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human BCMA, known as TNFRSF17, BCM, BCMA, CD269, TNFRSF13A, GenBank Accession No. NM_001192, a.a. 1-54, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 34 kDa.
Description: Human KIR2DL1, also known as Killer cell immunoglobulin-like receptor 2DL1, CD158A, or NKAT-1, GenBank Accession No. NM_014218, a.a. 22-242 fused at the C terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-tag™ and expressed in a HEK293 cell expression system. MW=53 kDa. This protein runs at a higher MW due to glycosylation.
Description: Human KIR2DL2, also known as Killer cell immunoglobulin-like receptor 2DL2, CD158K, or NKAT-4, GenBank Accession No. NM_014219, a.a. 22-245 fused at the C terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-tag™ and expressed in a HEK293 cell expression system. MW=53 kDa. This protein runs at a higher MW due to glycosylation.
Description: AIM-100 is a small inhibitor of Ack1 tyrosine kinase with IC50 value of 24 nM [1]. AIM-100 mimicked ATP and inhibited the activity of Ack1 significantly and specifically.
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.
Description: Human secreted CD44, also known as epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, or phagocytic glycoprotein I (PGP-1), GenBank Accession No. NM_000610, a.a. 21-220 expressed in a HEK293 cell expression system, fused at the C-terminus to the Fc portion of human IgG1, and enzymatically biotin-labeled using Avi-tag™ technology. MW = 50.6 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human secreted CD2-Fc fusion protein, also known as LFA-2, GenBank Accession No. NM_001767, a.a. 25-209, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, integrin-associated protein (IAP), OA3, and MER6, GenBank Accession No. NM_001777, a.a. 19-139, with C-terminal Avi-Tag, expressed in a HEK293 cell expression system. The purified protein (40 kDa) was covalently labeled by streptavidin (~13 kDa). Protein runs at a higher MW by SDS-PAGE due to glycosylation and streptavidin labeling.
Description: Mouse OX40, also known as Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_011659, a.a. 20-211 with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 52 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human Colony stimulating factor 1 receptor (CSF1R), also known as FMS and CD115, GenBank Accession No. NM_005211, a.a. 20-512, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW=83 kDa.
Description: Human CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 fused at the C-terminus to the Fc portion of human IgG1 and C-terminal Avi-tag expressed in a HEK293 cell expression system. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD112R, also known as Poliovirus Receptor-related Immunoglobulin Domain-containing Protein (PVRIG), GenBank Accession No. NM_024070, a.a. 41-172, with C-terminal Fc-Fusion-Avi-tag, expressed in a HEK293 cell expression system. MW=42 kDa.
Description: Human LILRB1, also known Leukocyte immunoglobulin-like receptor subfamily B member 1, CD85J, ILT2, and LIR-1, GenBank Accession No. NM_006669, a.a 24-458, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 76 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.
Description: Human ROR2, also known as tyrosine-protein kinase transmembrane receptor, neutrotrophic tyrosine kinase, and receptor related 2, GenBank Accession No. NM_004560, a.a. 28-403, with C-terminal Avi-Tag fused to the Fc region of Human IgG1, and expressed in a Hek293 expression system. MW=71 kDa.
Description: Human FGL1, also known as fibrinogen Like 1, liver fibrinogen-related protein 1, fibrinogen-like protein 1, or hepassocin, GenBank Accession No. NM_004467, a.a. 23-312 fused at the C terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-tag™ and expressed in a HEK293 cell expression system. MW=63 kDa.
Description: Recombinant human CD235a (also known as GYPA, glycophorin A), encompassing amino acids 20-91. The protein is fused in C-terminal with the Fc fragment of a human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
Description: Recombinant human CD235a (also known as GYPA, glycophorin A), encompassing amino acids 20-91. The protein is fused in C-terminal with the Fc fragment of a human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
Description: Recombinant human CD161 (also known as KLRB1, killer cell lectin like receptor B1), encompassing amino acids 67-225. The protein is fused at the N-terminus with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of Mouse IgG2a, expressed in a HEK293 cell expression system. MW = 43 kDa.
CD48, Fc Fusion (IgG1), Biotin-Labeled (Human) HiP™
Description: Human CD48, Fc fusion protein, also known as Cluster of Differentiation 48, BLAST-1, or SLAMF2. GenBank Accession No. NM_001778, a.a. 27-220 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 51 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
CD48, Fc Fusion (IgG1), Biotin-Labeled (Human) HiP™
Description: Human CD48, Fc fusion protein, also known as Cluster of Differentiation 48, BLAST-1, or SLAMF2. GenBank Accession No. NM_001778, a.a. 27-220 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 51 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD111, also known as Poliovirus receptor-related 1, netcin-1, herpesvirus entry mediator C, and HVEC. GenBank Accession No. NM_002855, a.a. 31-355, fused at the C-terminus to the Fc portion of Human IgG1 and with a C-terminal Avi-tag, expressed in a Hek293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW=65 kDa.
Description: Human CD111, also known as Poliovirus receptor-related 1, netcin-1, herpesvirus entry mediator C, and HVEC. GenBank Accession No. NM_002855, a.a. 31-355, fused at the C-terminus to the Fc portion of Human IgG1 and with a C-terminal Avi-tag, expressed in a Hek293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW=65 kDa.
Description: Mouse secreted CD27, Fc fusion protein, with C-terminal Avi-Tag, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001033126, a.a. 21-182 expressed in a HEK293 cell expression system. MW = 49 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Monkey CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. XM_015446485, a.a. 19-139, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human PDPN, known as Podoplanin, GenBank Accession No. NM_006474, a.a. 23-131, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 40 kDa.
Description: Human PDPN, known as Podoplanin, GenBank Accession No. NM_006474, a.a. 23-131, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 40 kDa.
Description: Human CD22 also known as cluster of differentiation-22, and SIGLEC-2, GenBank Accession No. NM_001771, a.a. 100-330 fused to Fc region of Human IgG1. This protein is enzymatically biotinylated using AviTag™ technology and expressed in a HEK293 cell expression system. MW = 104 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human CD22 also known as cluster of differentiation-22, and SIGLEC-2, GenBank Accession No. NM_001771, a.a. 100-330 fused to Fc region of Human IgG1. This protein is enzymatically biotinylated using AviTag™ technology and expressed in a HEK293 cell expression system. MW = 104 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Woodchuck secreted CTLA4, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152, with N-terminal Avi-TagTM fused to the Fc region of Human IgG1, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD33, also known as Sialic Acid Binding Ig Like Lectin 3, Gp67 and Myeloid Cell Surface Antigen CD33, GenBank Accession No. NM_001772, a.a. 1-259, fused at the C-terminus with the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. MW = 57 kDa. Biotinylation is confirmed to be ≥90%.
Description: Human secreted CD33, also known as Sialic Acid Binding Ig Like Lectin 3, Gp67 and Myeloid Cell Surface Antigen CD33, GenBank Accession No. NM_001772, a.a. 1-259, fused at the C-terminus with the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. MW = 57 kDa. Biotinylation is confirmed to be ≥90%.
Description: Human Erythropoietin Receptor, also known as EPOR, GenBank Accession No. NM_000121, a.a 25-250, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=54 kDa.
Description: Human Erythropoietin Receptor, also known as EPOR, GenBank Accession No. NM_000121, a.a 25-250, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW=54 kDa.
Description: Human transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_003242 (var2), a.a. 23-166 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW = 45 kDa. This protein runs at a higher molecular weight due to glycosylation.
Description: Human transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_003242 (var2), a.a. 23-166 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW = 45 kDa. This protein runs at a higher molecular weight due to glycosylation.
Description: Recombinant human CD5 encompassing amino acids 25-371. This protein is fused in C-terminal with the Fc fragment of human IgG1 (230 amino acids) followed by a C-terminal Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
Description: Recombinant human CD5 encompassing amino acids 25-371. This protein is fused in C-terminal with the Fc fragment of human IgG1 (230 amino acids) followed by a C-terminal Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
IL18R1, Fc Fusion (lgG1), Avi-Tag HiP™ Recombinant
Description: Recombinant human IL18R1 (Interleukin 18 receptor 1), encompassing amino acids 22-329. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was affinity purified.
Description: Recombinant human IL23R (interleukin 23 receptor), encompassing amino acids 24-355. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
IL18RAP, Fc Fusion (lgG1), Avi-Tag HiP™ Recombinant
Description: Recombinant human IL18RAP ( interleukin 18 receptor accessory protein), encompassing amino acids 20-356. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was affinity purified.
Description: For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.
Description: For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.
Description: For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD375nm. Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 12 µM.
Description: For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
Description: For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.
Description: For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.
Description: A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L.
Description: For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests.
Description: For quantitative determination of urokinase activity determination in biological samples. Key Features: Safe. Non-radioactive assay. Fast. Assay is completed within a 15 minute reaction time. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to assay thousands of samples per day. Method: FL380/450 nm; Samples: Biological Samples (e.g. Urine and serum). Species: All. Procedure: assay takes 15min. Size: 100 tests. Detection Limit: 0.04 U/L.
Description: For quantitative determination of creatinine and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. It is 3- and 53-fold more sensitive than the traditional Jaffe method (e.g. DICT-500), especially useful for small samples or where high sensitivity is required. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm; FL530/585nm. Samples: Urine, serum, plasma, and other biological preparations. Species: All. Procedure: 60 min. Size: 100 tests. Detection Limit: Colorimetric assay: 4.8 µM or 0.054 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL.
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274, B7 homolog 1 (B7- H1), and PD-L1 Biotin-Labeled. GenBank Accession No. NM_014143, amino acids 19-239, fused at the Cterminus with the Fc portion of human IgG1, with C-terminal Avi-tag™ expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 54 kDa.
PD-1 (CD279), Fc fusion, Biotin-labeled (Human) HiP™
Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279, HPD-L, PD-1 Biotin-labeled GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag ™ expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag™ technology. MW = 44.5 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™
Description: Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag™. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
PD-1 (CD279), Fc fusion, Biotin-labeled (Mouse) HiP™
Description: Mouse secreted Programmed Cell Death 1 (PD-1)-Fc fusion protein, also known as CD279, PDCD1, and SLEB2, GenBank Accession No. NM_008798, a.a. 25-167, fused at the C-terminus to the Fc portion of mouse IgG2a and with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein was biotinylated using Avi-Tag™ technology. MW = 45 kDa. Note: this protein runs at an apparent higher molecular weight due to glycosylation.
PD-L1 (CD274), Fc fusion, Biotin-labeled (Mouse) HiP™
Description: Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1) with a K124A mutation, GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus with the Fc portion of human IgG1, with C-terminal Avi-tag™ expressed in a HEK293 cell expression system. MW = 54 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Mouse TIM-3, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, HAVCR-2, and KIM-3. GenBank Accession No. NM_032782, a.a. 22-191, expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1. MW = 47 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
TIGIT, Fc Fusion (IgG1) Avi-Tag, Biotin (Mouse) HiP™
Description: Mouse T-cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, and VSTM3, GenBank Accession No. NM_001146325.1, a.a. 26-143 fused to Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
TIGIT, Fc Fusion (IgG1) Avi-Tag, Biotin (Mouse) HiP™
Description: Mouse T-cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, and VSTM3, GenBank Accession No. NM_001146325.1, a.a. 26-143 fused to Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Human secreted CD112R, also known as Poliovirus Receptor-related Immunoglobulin Domain-containing Protein (PVRIG), GenBank Accession No. NM_024070, a.a. 41-172, with C-terminal Fc-Fusion-Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag™ technology. MW = 42 kDa.
Description: Human secreted CD112R, also known as Poliovirus Receptor-related Immunoglobulin Domain-containing Protein (PVRIG), GenBank Accession No. NM_024070, a.a. 41-172, with C-terminal Fc-Fusion-Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag™ technology. MW = 42 kDa.
CD47 (Monkey), Fc Fusion (Human), Avi-Tag, Biotin HiP™
Description: Monkey CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. XM_015446485, a.a. 19-139, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. The protein is enzymatically biotinylated using AviTag™ technology. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
CD47 (Monkey), Fc Fusion (Human), Avi-Tag, Biotin HiP™
Description: Monkey CD47, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, and MER6. GenBank Accession No. XM_015446485, a.a. 19-139, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. The protein is enzymatically biotinylated using AviTag™ technology. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Description: Mouse OX40, also known as Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_011659, a.a. 20-211 with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 52 kDa.
Description: Mouse OX40, also known as Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_011659, a.a. 20-211 with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 52 kDa.
Description: Human BCMA also known as B-cell maturation antigen, tumor necrosis factor receptor superfamily member 17, and TNFRSF17, GenBank Accession No. NM_001192, a.a. 1-54 fused to Fc region of Human IgG1 with C-terminal Avi-Tag™, and expressed in a HEK293 cell expression system. MW = 34 kDa. This protein is enzymatically biotinylated using Avi-TagTM technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation._x000D_
Description: Human BCMA also known as B-cell maturation antigen, tumor necrosis factor receptor superfamily member 17, and TNFRSF17, GenBank Accession No. NM_001192, a.a. 1-54 fused to Fc region of Human IgG1 with C-terminal Avi-Tag™, and expressed in a HEK293 cell expression system. MW = 34 kDa. This protein is enzymatically biotinylated using Avi-TagTM technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation._x000D_
Description: Human LILRB1, also known Leukocyte immunoglobulin-like receptor subfamily B member 1, CD85J, ILT2, and LIR-1, GenBank Accession No. NM_006669, a.a 24-458, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 76 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology.
Description: Human LILRB1, also known Leukocyte immunoglobulin-like receptor subfamily B member 1, CD85J, ILT2, and LIR-1, GenBank Accession No. NM_006669, a.a 24-458, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. MW = 76 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation. This protein is enzymatically biotinylated using Avi-Tag™ technology.
Description: Human ROR2, also known as tyrosine-protein kinase transmembrane receptor, neutrotrophic tyrosine kinase, and receptor related 2, GenBank Accession No. NM_004560, a.a. 28-403, with C-terminal Avi-Tag fused to the Fc region of Human IgG1, and expressed in a Hek293 expression system. MW=71 kDa. This protein is enzymatically biotinylated using Avi-TagTM technology.
TAC1, Fc Fusion (IgG1) Avi-Tag, Biotin Labeled, HiP™
Description: Human TAC1, also known as Hs.2563, NK2, NKNA, NPK, TAC2, and tachykinin precursor 1. GenBank Accession No. NM_0012452, a.a. 23-166, fused at the C-terminus of the Fc portion of Human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW = 48 kDa.
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