Antibodies, Assay Kits, Brd4 Antibody, cDNA, Clia Kits, Ctip Antibody, Culture Cells, Gels, Isotypes, Nedd8 Antibody, Nmnat1 Antibody, Nse Antibody, Otx2 Antibody, Particles, PCR, Pcr Kits, RNA, Setd2 Antibody, Sod2 Antibody, Stat5 Antibody, Trpa1 Antibody, Vector & Virus

Analysis of the reliability of patient COVID-19 screening data compared against their actual test data

Goals: This examine aimed to judge the truthfulness of sufferers about their pre-appointment COVID-19 screening assessments at a dental clinic.
Strategies: A complete of 613 sufferers have been recruited for the examine from the dental clinic on the College of Dentistry, Najran College, Saudi Arabia. The information assortment was performed in three components from the sufferers who visited the hospital to obtain dental therapy. The primary half included the socio-demographic traits of the sufferers and the COVID-19 swab assessments carried out throughout the previous 14 days. The second half was the medical examination, and the third half was a affirmation of the swab check taken by the affected person by checking the Hesen web site utilizing the affected person ID. After knowledge assortment, statistical evaluation was carried out utilizing SPSS 26.0. Descriptive evaluation was performed and expressed as imply, normal deviation, frequency, and share (%). A cross-tabulation, additionally described as a contingency desk, was used to determine tendencies and patterns throughout knowledge and clarify the correlation between completely different variables.
Outcomes: It was seen from the standing of the swab check inside 14 days of the affected person’s arrival on the hospital for the dental therapy that 18 (2.9%) sufferers lied in regards to the pre-treatment swab check inside 14 days, and 595 (97.1%) have been truthful. The noticed and anticipated counts confirmed throughout genders and analysis a statistically important distinction (p < 0.001), and there was no important distinction seen throughout completely different age teams (p = 0.064) of the sufferers.
Conclusions: Dental healthcare employees are nervous and assume a excessive danger of COVID-19 an infection because the sufferers are usually not truthful in regards to the pre-treatment COVID-19 swab check. Routine speedy assessments on sufferers and the healthcare workers are a possible possibility for reducing general dangers.
milansystem
milansystemmilansystem

cDNA cloning, expression, and antifungal exercise of chitinase from Ficus microcarpa latex: distinction in antifungal motion of chitinase with and with out chitin-binding area

 

A chitin-binding area might contribute to the antifungal means of chitinase via its affinity to the fungal lateral wall by hydrophobic interactions. Complementary DNA encoding the antifungal chitinase of gazyumaru (Ficus microcarpa), designated GlxChiB, was cloned and expressed in Escherichia coli cells. The outcomes of cDNA cloning confirmed that the precursor of GlxChiB has an N-terminal endoplasmic reticulum focusing on sign and C-terminal vacuolar focusing on sign, whereas mature GlxChiB consists of an N-terminal carbohydrate-binding module family-18 area (CBM18) and a C-terminal glycoside hydrolase family-19 area (GH19) with a brief linker. To make clear the function of the CBM18 area within the antifungal exercise of chitinase, the recombinant GlxChiB (wild sort) and its catalytic area (CatD) have been used in quantitative antifungal assays below completely different ionic strengths and microscopic observations towards the fungus Trichoderma viride.

The antifungal exercise of the wild sort was stronger than that of CatD below all ionic energy circumstances used on this assay; nevertheless, the antifungal exercise of CatD grew to become weaker with growing ionic energy, whereas that of the wild sort was maintained. The outcomes at excessive ionic energy additional verified the contribution of the CBM18 area to the antifungal means of GlxChiB. The microscopic observations clearly confirmed that the wild sort acted on each the ideas and the lateral wall of fungal hyphae, whereas CatD acted solely on the ideas. These outcomes recommend that the CBM18 area might contribute to the antifungal means of chitinase via its affinity to the fungal lateral wall by hydrophobic interactions

 

Utility of the modified handmade cloning method to pigs

Though somatic cell nuclear switch (SCNT) is often employed to provide cloned animals in laboratories, this system is pricey and inefficient. Subsequently, the handmade cloning (HMC) method has been urged to simplify and advance the cloning course of, nevertheless, HMC wastes many oocytes and results in mitochondrial heteroplasmy. To resolve these issues, we suggest a modified handmade cloning (mHMC) method that makes use of easy laboratory tools, i.e., a Pasteur pipette and an alcohol lamp, making use of it to porcine embryo cloning. To validate the utility of mHMC to pig cloning, embryos produced via SCNT and mHMC are in contrast utilizing a number of strategies, equivalent to enucleation effectivity, oxidative stress, embryo developmental competence, and gene expression.

The outcomes present no important variations between strategies besides within the enucleation effectivity. The 8-cell and 16-cell embryo developmental competence and Oct4 expression ranges exhibit important variations. Nevertheless, the blastocyst charge just isn’t considerably completely different between mHMC and SCNT. This examine verifies that cloned embryos derived from the 2 strategies exhibit related technology and developmental competence. Thus, we propose that mHMC might exchange SCNT for easier and cheaper porcine cloning.

Molecular cloning and characterization of high-affinity potassium transporter (AlHKT2;1) gene promoter from halophyte Aeluropus lagopoides

HKT subfamily II capabilities as Na+– Ok+ co-transporter and prevents crops from salinity stress. A 760 bp promoter area of AlHKT2;1 was remoted, sequenced and cloned. The total size promoter D1, has many cis-regulatory parts like MYB, MBS, W field, ABRE and many others. concerned in abiotic stress responses. D1 and subsequent 5′ deletions have been cloned into pCAMBIA1301 and studied for its efficacy in stress circumstances in heterologous system. Blue color staining was noticed in flower petals, anther lobe, and dehiscence slit of anther in T0 crops. The T1 seedling confirmed staining in leaf veins, shoot vasculature and root besides root tip. T1 seedlings have been subjected to NaCl, KCl and NaCl + KCl and ABA stresses. GUS exercise was quantified by 4-methylumbelliferyl glucuronide (4-MUG) assay below management and stress circumstances.

The smallest deletion- D4 additionally confirmed GUS expression however highest exercise was noticed in D2 as in comparison with full size promoter and different deletions. The electrophoretic mobility shift assay utilizing stress-induced protein with completely different promoter deletions revealed extra outstanding binding in D2. These outcomes recommend that AlHKT2;1 promoter is concerned in abiotic stress response and deletion D2 is perhaps ample to drive the stress-inducible expression of assorted genes concerned in offering stress tolerance in crops

Stem cell therapies and benefaction of somatic cell nuclear switch cloning in COVID-19 period

Background: The worldwide well being emergency of COVID-19 has necessitated the event of a number of therapeutic modalities together with vaccinations, antivirals, anti-inflammatory, and cytoimmunotherapies, and many others. COVID-19 sufferers endure from harm to varied organs and vascular buildings, so that they current a number of well being crises. Mesenchymal stem cells (MSCs) are of curiosity to deal with acute respiratory misery syndrome (ARDS) attributable to SARS-CoV-2 an infection.

Important physique: Stem cell-based therapies have been verified for potential advantages in copious preclinical and medical research. MSCs confer potential advantages to develop numerous cell varieties and organoids for finding out virus-human interplay, drug testing, regenerative drugs, and immunomodulatory results in COVID-19 sufferers. Aside from paving the methods to reinforce stem cell analysis and therapies, somatic cell nuclear switch (SCNT) holds distinctive means for a wide selection of well being purposes equivalent to patient-specific or isogenic cells for regenerative drugs and breeding transgenic animals for biomedical purposes. Being a potent cell genome-reprogramming instrument, the SCNT has elevated prominence of recombinant therapeutics and mobile drugs within the present period of COVID-19. As SCNT is used to generate patient-specific stem cells, it avoids dependence on embryos to acquire stem cells.

Conclusions: The nuclear switch cloning, being a really perfect instrument to generate cloned embryos, and the embryonic stem cells will increase drug testing and mobile drugs in COVID-19.

Molecular cloning of duck CD40 and its immune operate analysis

Cosignal molecules are cell floor molecules that transduce alerts to different cells to modulate immune response positively (costimulate) or negatively (cosuppress). Costimulatory alerts are key elements in figuring out whether or not T/B cells are able to responding to particular antigens and in the end mediating an applicable immune response. On this examine, the cDNA sequence containing the whole coding body of the costimulatory molecule duck CD40 gene was cloned and reported for the primary time, and its mediated antiviral innate immune was verified in vitro. Outcomes urged duck CD40 molecule performs an vital function within the innate immune responsiveness towards some viruses. These knowledge will likely be useful for the additional perceive of the avian immune system.

Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Utility within the Deconstruction of Corn Stover

Xylanase performs an important function within the environment friendly utilization of xylan, which accounts for as much as 30% of plant dry matter. Nevertheless, the manufacturing value of xylanase stays excessive, and the enzymatic traits of xylanases of most microorganisms are usually not appropriate for industrial manufacturing. Subsequently, it’s of nice significance to find and develop new and environment friendly xylanases. On this examine, the xylanase gene TAX1 (672 bp cDNA) was cloned from Trichoderma atroviride 3.3013 and expressed in Pichia pastoris. The TAX1 gene encoded a 223-amino acid protein (TAX1) with a molecular weight of 24.2 kDa which confirmed excessive similarity to glycoside hydrolase household 11. Enzyme exercise assay verified that the recombinant xylanase TAX1 had optimum exercise (215.Three IU/mL) at 50°C and pH 6.0.

Steady working circumstances have been measured as pH 4.0-7.Zero and 40-60°C. By including Zn2+, the relative enzymatic exercise of recombinant TAX1 was enhanced by 26%. The recombinant xylanase confirmed excessive exercise towards birchwood xylan and corn stover. The Okm and Okcat for xylan and corn stover have been 0.36 mg/mL and 0.204 S-1 and 0.48 mg/mL and 0.149 S-1, respectively. The enzymatic exercise of the TAX1 produced by P. pastoris was about 2.4-Four occasions increased that straight remoted from T. atroviride, so engineered P. pastoris for xylanase manufacturing may very well be a really perfect candidate for industrial enzyme manufacturing.

igScript One Step RT-qPCR Kit - 100 Reactions

4214 1/EA
EUR 303.6

Accuris One-Step RT-PCR kit, 100 reactions

PR1100-100 1 each
EUR 509.6

illustra Hot Start Master Mix 100 reactions

25150001 EACH
EUR 162

igScript First Strand cDNA Synthesis Kit - 100 Reactions

4314 1/EA
EUR 453.6

Accuris qMax Green, No Rox qPCR Mix, 100 reactions

PR2000-N-100 1 PC
EUR 77.7

Accuris qMax Probe, No Rox qPCR Mix, 100 reactions

PR2001-N-100 1 PC
EUR 77.7

Accuris qMax Green, Low Rox qPCR Mix, 100 reactions

PR2000-L-100 1 PC
EUR 77.7

Accuris qMax Probe, Low Rox qPCR Mix, 100 reactions

PR2001-L-100 1 PC
EUR 77.7

Accuris qMax Green, High Rox qPCR Mix, 100 reactions

PR2000-H-100 1 PC
EUR 77.7

Accuris qMax Probe, High Rox qPCR Mix, 100 reactions

PR2001-H-100 1 PC
EUR 77.7

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)

31041-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 100 Reactions

30101-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 100 Reactions

30102-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 100 Reactions

30103-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 100 Reactions

30104-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Forget-Me-Notâ„¢ Universal Probe Master Mix (100 reactions)

31043-T 1kit
EUR 136.8
Description: Minimum order quantity: 1 unit of 1kit

Accuris qMAX Green One-Step RT-qPCR Kit,No Rox, 100 reactions

PR2120-N-100 1 each
EUR 175.4

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 100 reactions

PR2121-N-100 1 each
EUR 175.4

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 reactions

PR2120-L-100 1 each
EUR 175.4

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions

PR2121-L-100 1 each
EUR 175.4

igScript RT-PCR kit - 200 Reactions

4415 1/EA
EUR 796.8

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 100 reactions

PR2120-H-100 1 each
EUR 175.4

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 100 reactions

PR2121-H-100 1 each
EUR 175.4

i7 High-Fidelity DNA Polymerase 2X Master Mix - 100 Reactions

3257 1/EA
EUR 234

igScript RT-PCR kit - 50 Reactions

4413 1/EA
EUR 259.2

GFP-nAb Magnetic Kit (20 Reactions)

ABP-NAB-GFPMK20 20 Reactions Ask for price

RFP-nAb Magnetic Kit (20 Reactions)

ABP-NAB-RFPMK20 20 Reactions Ask for price

ACTaq? Mastermix 2x, 200 reactions

E2110-200RXN each
EUR 204

Pfu 2x Master Mix - 200 Reactions

3326 1/EA
EUR 360

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions)

31042-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

FastAmp Plant Direct PCR Kit - 1250 Reactions

4615 1/EA
EUR 621.6

GFP-nAb? Agarose Spin Kit (20 Reactions)

ABP-NAB-GFPAK20 20 Reactions Ask for price

RFP-nAb Agarose Spin Kit (20 Reactions)

ABP-NAB-RFPAK20 20 Reactions Ask for price

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 100 reactions

PR2122-N-100 1 each
EUR 182.42

mNeonGreen-nAb Agarose Kit (20 Reactions)

ABP-NAB-MNGAK20 20 Reactions Ask for price

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (100 reactions)

31044-T 1kit
EUR 136.8
Description: Minimum order quantity: 1 unit of 1kit

ACTaq? Mastermix 2x, 50 reactions

E2110-50RXN each
EUR 104.4

Mouse Tail DNA Extraction Kit (250 reactions)

T605 - Ask for price

mTFP-nAb Agarose Spin Kit (20 Reactions)

ABP-NAB-MTFPAK20 20 Reactions Ask for price

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

Genorise® Cell Viability Kit (2,000 reactions)

GR110001 2,000 tests
EUR 598

Genorise® Cell Viability Kit (10,000 reactions)

GR110001-10 10,000 tests
EUR 1980

Genorise® Cell Viability Kit (5,000 reactions)

GR110001-5 5,000 tests
EUR 1100

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1078

Accuris qMax cDNA Synthesis Kit, 250 reactions

PR2100-C-250 1 PC
EUR 1021.6

igScript One Step RT-PCR Kit - 500 Reactions

4213 1/EA
EUR 908.4

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 275

GFP-nAb Magnetic Agarose Kit (20 Reactions)

ABP-NAB-GFPXK20 20 Reactions Ask for price

RFP-nAb Magnetic Agarose Kit (20 Reactions)

ABP-NAB-RFPXK20 20 Reactions Ask for price

RNA-Quant cDNA synthesis Kit (20 reactions)

RA430A-1 20 reactions
EUR 619

i7 Hot Start High-Fidelity DNA Polymerase 2X Master Mix - 100 Reactions

3284 1/EA
EUR 266.4

GenomiPhi HY DNA Amplification Kit 1000 reactions

25660025 EACH
EUR 19711.2

Accuris qMax cDNA Synthesis Kit, 25 reactions

PR2100-C-25 1 PC
EUR 122.4

Accuris One-Step RT-PCR kit, 50 reactions

PR1100-50 1 each
EUR 282.5

Cold Fusion Cloning Kit with Competent Cells (10 rxns) International Sales Only

MC010A-1 10 reactions
EUR 251

Cold Fusion Cloning Kit with Competent Cells (20 rxns) International Sales Only

MC100A-1 20 reactions
EUR 459

Cold Fusion Cloning Kit with Competent Cells (50 rxns) International Sales Only

MC101A-1 50 reactions
EUR 1056

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 228

ExoMAX Opti Enhancer Reagent (12 reactions)

EXOMAX12A-1 12 reactions
EUR 389

ExoMAX Opti Enhancer Reagent (24 reactions)

EXOMAX24A-1 24 reactions
EUR 584

pPACKF1 FIV Lentivector Packaging Kit (50 reactions)

LV100A-5 50 transfections
EUR 2240

mTFP-nAb Magnetic Agarose Spin Kit (20 Reactions)

ABP-NAB-MTFPXK20 20 Reactions Ask for price

Accuris 1 Hour Mammalian Genotyping Kit, 400 Reactions

PR1300-MG-400 1 PC
EUR 600.3

Accuris Taq Master Mix, 2X conc., 1000 reactions

PR1001-1000 1 PC
EUR 364.9

Accuris Taq Master Mix, 2X conc., 200 reactions

PR1001-200 1 PC
EUR 87.1

Accuris qMax cDNA Synthesis Kit, sample, 5 reactions

PR2100-C-S 1 PC
EUR 18.8

Accuris One-Step RT-PCR kit, sample 10 reactions

PR1100-S 1 each
EUR 60

Accuris qMAX Probe, No ROX, 2500 reactions (1x25ml)

PR2001-N-25BLK 1 each
EUR 1133

Accuris qMAX Probe, No ROX, 5000 reactions (1x50ml)

PR2001-N-50BLK 1 each
EUR 2039.4

6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*

398 5 ml
EUR 334

igScript First Strand cDNA Synthesis Kit - 25 Reactions

4312 1/EA
EUR 178.8

Accuris 1 Hour Mammalian Genotyping Kit, 80 Reactions

PR1300-MG-80 1 PC
EUR 137.7

illustra GenomiPhi V2 DNA Amplification Kit 500 reactions

25660032 EACH
EUR 2622

Exosome ELISA Complete Kit (CD9 detection) 96 reactions

EXOEL-CD9A-1 96 reactions
EUR 956.4

Accuris qMax Green, No Rox qPCR Mix, 1000 reactions

PR2000-N-1000 1 PC
EUR 623.8

Accuris qMax Probe No Rox qPCR Mix, 1000 reactions

PR2001-N-1000 1 PC
EUR 623.8

Accuris qMax Green, No Rox qPCR Mix, 500 reactions

PR2000-N-500 1 PC
EUR 340.2

Accuris qMax Probe, No Rox qPCR Mix, 500 reactions

PR2001-N-500 1 PC
EUR 340.2

SeraMir 3'-Universal Primer (10,000 qPCR reactions)

RA800P-3 10,000 reactions
EUR 125

Accuris Taq Plus Master Mix, 2X conc., 1000 reactions

PR1001-TP-1000 1 PC
EUR 1182.9

Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions

PR2000-L-1000 1 PC
EUR 623.8

Accuris qMax Probe, Low Rox qPCR Mix, 1000 reactions

PR2001-L-1000 1 PC
EUR 623.8

Accuris Taq Plus Master Mix, 2X conc., 200 reactions

PR1001-TP-200 1 PC
EUR 276.6

Accuris qMax Green, Low Rox qPCR Mix, 500 reactions

PR2000-L-500 1 PC
EUR 340.2

Accuris qMax Probe, Low Rox qPCR Mix, 500 reactions

PR2001-L-500 1 PC
EUR 340.2

Accuris qMax Green, High Rox qPCR Mix, 1000 reactions

PR2000-H-1000 1 PC
EUR 623.8

Accuris qMax Probe, High Rox qPCR Mix, 1000 reactions

PR2001-H-1000 1 PC
EUR 623.8

Accuris qMax Green, High Rox qPCR Mix, 500 reactions

PR2000-H-500 1 PC
EUR 340.2

Accuris qMax Probe, High Rox qPCR Mix, 500 reactions

PR2001-H-500 1 PC
EUR 340.2

QuantiMir 3'-Universal Primer (10,000 qPCR reactions)

RA420AU-3 10,000 assays
EUR 125

Accuris qMAX SYBR Green, No ROX, 2500 reactions (1x25ml)

PR2000-N-25BLK 1 each
EUR 1133

Accuris qMAX SYBR Green, No ROX, 5000 reactions (1x50ml)

PR2000-N-50BLK 1 each
EUR 2039.4

Accuris Taq Master Mix Red Dye, 2X conc., 1000 reactions

PR1001-R-1000 1 PC
EUR 364.9

Accuris Taq Master Mix Red Dye, 2X conc., 200 reactions

PR1001-R-200 1 PC
EUR 88.3

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 200 reactions

PR2110-200 1 PC
EUR 422.5

Cold Fusion Cloning Kit [96X] with 96-well format for Competent Cells -- International Sales Only

MC096A-1 10 reactions
EUR 1856

Accuris Hot Start Taq Master Mix, 2X conc., 1000 reactions

PR1001-HS-1000 1 PC
EUR 659.1

Accuris 1 Hour Mammalian Genotyping Kit, Sample, 8 reactions

PR1300-MG-S 1 PC
EUR 9.4

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 1000 reactions 

PR2120-N-1000 1 each
EUR 1398.3

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 1000 reactions

PR2121-N-1000 1 each
EUR 1398.3

Accuris Taq Master Mix, 2X conc., sample, 5 reactions

PR1001-S 1 PC
EUR 7.1

Accuris Hot Start Taq Master Mix, 2X conc., 200 reactions

PR1001-HS-200 1 PC
EUR 161.2

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 500 Reactions

30101 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 500 Reactions

30102 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 500 Reactions

30103 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 500 Reactions

30104 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, 500 reactions

PR2120-N-500 1 each
EUR 762.7

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 500 reactions

PR2121-N-500 1 each
EUR 762.7

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 reactions

PR2120-L-1000 1 each
EUR 1398.3

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 1000 reactions

PR2121-L-1000 1 each
EUR 1398.3

PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit-96 reactions

78326-1 96 reactions
EUR 750
Description: The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Accuris Hot Start Taq Mastermix, sample, 5 reactions

PR1001-HS-S 1 PC
EUR 7.1

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 500 reactions

PR2120-L-500 1 each
EUR 762.7

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 500 reactions

PR2121-L-500 1 each
EUR 762.7

PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit-384 reactions

78326-2 384 reactions
EUR 1150
Description: The PCSK9(D374T) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9(D374T) by streptavidin-HRP. Only a few steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9(D374T) is incubated with LDLR on the plate.  Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 1000 reactions 

PR2120-H-1000 1 each
EUR 1398.3

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 1000 reactions

PR2121-H-1000 1 each
EUR 1398.3

Accuris qMAX First Strand cDNA Synthesis Flex Kit, 50 reactions

PR2110-50 1 PC
EUR 104.8

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)

31041-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 500 reactions

PR2120-H-500 1 each
EUR 762.7

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 500 reactions

PR2121-H-500 1 each
EUR 762.7

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)

31041-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

IL-6 (Human) Colorimetric ELISA Detection Kit -5 x 96 reactions

79820-2 5 x 96 rxns.
EUR 610
Description: Interleukin-6 is a cytokine produced in response to infections or tissue damage that plays an important role in inflammation, immune response and hematopoiesis. The Colorimetric Human IL-6 Detection Kit is designed for detecting and quantifying human interleukin-6 in cell culture medium. This kit comes in a convenient 96-well format, with capturing and detection antibodies for IL-6, streptavidin-labeled HRP, blocking buffer, IL-6 standard, and colorimetric HRP substrate for a 96-well plate. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-6 are incubated on the coated plate followed by detecting the captured IL-6 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Amersham Rediprime II DNA Labeling System 30 reactions

RPN1633 EACH
EUR 740.4

Accuris qMax Green, No Rox qPCR Mix, sample, 20 reactions

PR2000-N-S 1 PC
EUR 12.9

Accuris qMax Probe, No Rox qPCR Mix, sample, 20 reactions

PR2001-N-S 1 PC
EUR 12.9

Forget-Me-Notâ„¢ Universal Probe Master Mix (500 reactions)

31043-1 1kit
EUR 396
Description: Minimum order quantity: 1 unit of 1kit

Accuris Taq Plus Master Mix, 2X conc., sample, 20 reactions

PR1001-TP-S 1 PC
EUR 7.1

Accuris qMax Green, Low Rox qPCR Mix, sample, 20 reactions

PR2000-L-S 1 PC
EUR 12.9

Accuris qMax Probe, Low Rox qPCR Mix, sample, 20 reactions

PR2001-L-S 1 PC
EUR 12.9

Accuris qMax Green, High Rox qPCR Mix, sample, 20 reactions

PR2000-H-S 1 PC
EUR 12.9

Accuris qMax Probe, High Rox qPCR Mix, sample, 20 reactions

PR2001-H-S 1 PC
EUR 12.9

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 1000 reactions

PR1001-HSR-1000 1 PC
EUR 659.1

i7 High-Fidelity DNA Polymerase 2X Master Mix - 500 Reactions

3259 1/EA
EUR 720

Accuris Probe One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

PR2121-N-25BLK 1 each
EUR 2695

Accuris Probe One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

PR2121-N-50BLK 1 each
EUR 4785

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 200 reactions

PR1001-HSR-200 1 PC
EUR 161.2

Antibody/Protein conjugation to FITC kit (sufficient for 2 reactions)

80230 1 kit
EUR 534

Accuris Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

PR1001-R-S 1 PC
EUR 7.1

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 1000 reactions

PR2122-N-1000 1 each
EUR 1454.23

Accuris qMAX First Strand cDNA Synthesis Flex Kit, sample, 5 reactions

PR2110-S 1 PC
EUR 11.8

Accuris qMAX Green One-Step RT-qPCR Kit, No Rox, sample 20 reactions

PR2120-N-S 1 each
EUR 18.8

Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, sample 20 reactions

PR2121-N-S 1 each
EUR 18.8

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 500 reactions

PR2122-N-500 1 each
EUR 793.21

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

PR2120-L-S 1 each
EUR 18.8

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, sample 20 reactions

PR2121-L-S 1 each
EUR 18.8

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)

9-31045
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

CyDye DIGE Fluor Labeling Kit for Scarce Samples 12 labelling reactions

25800984 EACH
EUR 2724

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

PR2120-N-25BLK 1 each
EUR 2695

Accuris SYBR Green One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

PR2120-N-50BLK 1 each
EUR 4785

Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, sample 20 reactions

PR2120-H-S 1 each
EUR 18.8

Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, sample 20 reactions

PR2121-H-S 1 each
EUR 18.8

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions)

31042-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions)

31042-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions)

9-31046
  • EUR 136.80
  • EUR 578.40
  • EUR 319.20
  • 1mL
  • 1X10mL
  • 5x1mL
Description: Minimum order quantity: 1 unit of 5x1mL

BasicExoRNA? Basic RNA extraction kit (Pre-isolated exosomes, 25 reactions)

K1222-25 each
EUR 874.8

BasicExoRNA? Basic RNA extraction kit (Pre-isolated exosomes, 50 reactions)

K1222-50 each
EUR 1462.8

Forget-Me-Notâ„¢ Universal Probe Master Mix with ROX (500 reactions)

31044-1 1kit
EUR 396
Description: Minimum order quantity: 1 unit of 1kit

5-ROX glycine *Fluorescence reference standard for PCR reactions*

396 25 mg
EUR 367.2

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 2500 reactions (1x25ml, 2x1.25ml)

PR2122-N-25BLK 1 each
EUR 2805

Accuris Probe Multiplex One-Step RT-qPCR Kit, No ROX, 5000 reactions (1x50ml, 4x1.25ml)

PR2122-N-50BLK 1 each
EUR 5049

i7 Hot Start High-Fidelity DNA Polymerase 2X Master Mix - 500 Reactions

3286 1/EA
EUR 819.6

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

PR1001-HSR-S 1 PC
EUR 7.1

6-ROXtraâ„¢ fluorescence reference solution *25 uM for PCR reactions*

398-5ml 5 ml
EUR 334
Description: 6-ROX is predominately used as a reference dye for performing PCR detections.

Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, sample 20 reactions

PR2122-N-S 1 each
EUR 19.55

pPACK-ID: Integrase-defective lentiviral packaging mix (15 reactions)

LV520A-ID 15 reactions
EUR 633

pPACK-ID: Integrase-defective lentiviral packaging mix (30 reactions)

LV525A-ID 30 reactions
EUR 1058

Peptide/Protein conjugation to Pre-activated BSA Kit (sufficient for 4 reactions)

80240 1 kit
EUR 489.6

Peptide/Protein conjugation to Pre-activated KLH Kit (sufficient for 4 reactions)

80250 1 kit
EUR 489.6

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

395 5 ml
EUR 222

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

395-5ml 5 ml
EUR 222
Description: 6-ROX is predominately used as a reference dye for performing PCR detections.

ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

400 5 mL
EUR 222

ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

400-5mL 5 mL
EUR 222
Description: ROX Reference Dye is equivalent to 12223-012 (Life Technology).

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (10 reactions)

K1221-10 each
EUR 992.4

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (20 reactions)

K1221-20 each
EUR 1462.8

Peptide/Protein conjugation to Pre-activated ovalbumin, Kit (sufficient for 4 reactions)

80260 1 kit
EUR 489.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1030-10 each
EUR 1005.6

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1030-20 each
EUR 1546.8

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1030-3 each
EUR 529.2

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1030-5 each
EUR 796.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1031-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1031-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)

M1031-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 5 reactions)

M1031-5 each
EUR 796.8

Custom Production of Monoclonal antibodies (immunization, ELISA Kit, fusion, screening, Cloning of up to 3 clones)

MAB-1 1
EUR 12483.6

Ready-to-go(TM) Genomiphi(TM) V3 DNA Amplification Kit; Cytiva; 25-6601-96; sufficient for 96 reactions

GE25-6601-96 PK96
EUR 681.6

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)

K1230-20 each
EUR 861.6

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 40 reactions)

K1230-40 each
EUR 1273.2

ExoDNAUC? Circulating and Exosome-associated DNA Extraction Kit (Urine/Cell Media, 20 reactions)

K1231-20 each
EUR 861.6

ExoDNAUC? Circulating and Exosome-associated DNA Extraction Kit (Urine/Cell Media, 40 reactions)

K1231-40 each
EUR 1273.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

M1039-10 each
EUR 1005.6

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)

M1039-20 each
EUR 1546.8

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)

M1039-3 each
EUR 523.2

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 5 reactions)

M1039-5 each
EUR 796.8

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1032-10 each
EUR 1057.2