Particles, Recombinant Proteins, RNA

Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control.

Unfavorable course of situations result in hostile cultivation states, restricted cell development and thus hamper biotherapeutic protein manufacturing.
Oxygen deficiency or hyperosmolality are among the many most crucial course of situations and due to this fact require steady monitoring.
We established a novel sensor CHO cell line with the power to robotically sense and report undesirable course of situations by the expression of destabilized fluorescent proteins.
To this finish, an inducible real-time system to detect hypoxia by hypoxia response parts (HREs) of vascular endothelial development issue (VEGF) origin reporting limitations by the expression of destabilized inexperienced fluorescent protein (GFP) was created.
Moreover, we established a way for observing hyperosmolality by exploiting osmotic response parts (OREs) for the expression of unstable blue fluorescent protein (BFP, FKBP-BFP), enabling the simultaneous automated supervision of two bioprocess parameters through the use of a twin sensor CHO cell line transfected with a multiplexable monitoring system.
We lastly additionally offered a completely automated in-line fluorescence microscopy-based setup to look at CHO cells and their response to various tradition situations.
In abstract, we created the primary CHO cell line, reporting unfavorable course of parameters to the operator, and offered a novel and promising sensor know-how accelerating the implementation of the method analytical know-how (PAT) initiative by revolutionary options.