Power and sample size calculation for the win odds test: application to an ordinal endpoint in COVID-19 trials
Precise-world medical effectivity of financial SARS-CoV-2 quick antigen exams in suspected COVID-19: A scientific meta-analysis of accessible information as per November 20, 2020
Goals: Speedy antigen exams, or RATs, are a sort of lateral circulation chromatographic immunoassay which had been utilized to assist prognosis of SARS-CoV-2 an an infection. We carried out a scientific meta-analysis to match the real-world effectivity of commercially obtainable RATs.
Methods: We searched various databases and internet sites for manufacturer-independent potential medical effectivity analysis evaluating SARS-CoV-2 RATs and RT-PCR. Solely analysis on RATs that did not need a separate reader for final result retrieval and that reported information on viral load, victims’ symptom standing, sample variety, and PCR assay used had been included.
Outcomes: 19 analysis utilizing 11,109 samples with 2,509 RT-PCR-positives had been included. RAT sensitivity completely different between 28.9% (95% CI 16.4-44.3) and 98.3% (95% CI 91.1-99.7), probably dependent upon inhabitants traits, viral load, and symptom standing.
RAT specificity completely different between 92.4% (95% CI 87.4-95.9) and 100% (95% CI 99.7-100) with one outlier. The RATs by Roche Diagnostics/SD Biosensor and Abbott had the perfect pooled sensitivity (82.4% [95% CI 74.2-88.4] and 76.9% [95% CI 72.1-81.2], respectively). Sensitivity in high-viral-load samples (cycle threshold ≤25) confirmed heterogeneity among the many many completely completely different RATs.
Conclusion: The RATs supplied by Roche Diagnostics/SD Biosensor and Abbott current ample manufacturer-independent, real-world effectivity information to assist their use for detection of current SARS-CoV-2 an an infection, notably in high-viral-load populations.
Key phrases: COVID-19; SARS-CoV-2; immunoassay; lateral circulation; point-of-care testing; quick antigen examine.
Evaluation of Triage Checks When Present Test Functionality Is Constrained: Utility to Speedy Diagnostic Testing in COVID-19
Goals: A triage examine is used to seek out out which victims will endure an present or “reference” examine. This textual content explores the potential price of using triage exams sooner than reference exams when the aptitude of the reference examine is constrained.
Methods: We developed a simple model with inputs: prevalence, sensitivity, specificity, and reference examine functionality. We included a case analysis of quick diagnostic exams for SARS-CoV-2 antigens used as triage exams sooner than a reference polymerase chain response examine. Effectivity information had been obtained from an evaluation carried out by an academic coronary heart on 425 samples from testing services within the UK and Germany.
Outcomes: When reference examine functionality is constrained, the utilization of a triage examine leads to a relative progress of the inhabitants examined and circumstances acknowledged; every are bigger with a high-specificity triage examine.
When reference examine functionality is simply not constrained, the potential advantages of introducing a triage examine can be assessed using a typical cost-utility framework, balancing the utility of the low cost inside the number of reference exams required in direction of the disutility of missed circumstances associated to the utilization of a lower-sensitivity triage examine.
Inside the constrained case, the advantage of a triage testing approach by means of inhabitants lined and circumstances acknowledged is lowered as a result of the prevalence will improve. Inside the unconstrained case, the low cost in reference exams required is lowered and the number of circumstances missed improve as a result of the prevalence rises.
Conclusion: When the supply of the reference examine is constrained, exams added in a triage place don’t desire extreme ranges of accuracy to increase the number of circumstances acknowledged. This has implications in numerous sickness areas, along with COVID-19.
Twitter: Lower-accuracy #COVID19 exams can nonetheless be useful, based mostly on a model new article, “Evaluation of Triage Checks When Present Test Functionality Is Constrained: Utility to Speedy Diagnostic Testing in COVID-19,” co-authored by @janetbouttell & @NeilSHawkins @HEHTAGlasgow @MedDecMak There could also be loads emphasis on high-accuracy #COVID19 exams, nevertheless lower-accuracy exams have their place, based mostly on a model new analysis on triage exams when examine functionality is constrained, co-authored by @janetbouttell & @NeilSHawkins @HEHTAGlasgow @MedDecMak A model new analysis, co-authored by @janetbouttell & @NeilSHawkins @HEHTAGlasgow, has confirmed that when there’s restricted functionality of high-accuracy #COVID19 diagnostic exams, triage testing strategies can improve the inhabitants lined and the number of #COVID19 circumstances acknowledged.
Speedy COVID-19 antigenic exams: Usefulness of a modified approach for prognosis
Background: The current reliable actually useful examine for COVID-19 prognosis is RT-qPCR. Speedy antigen examine items could very properly be useful as they’re cheap, sooner with out the need of specialized laboratories to hold out the examine.
We report the performances of two quick immunochromatographic antigen testing items as compared with RT-qPCR for SARS-CoV-2 detection in medical samples.
Methods: We carried out a lateral-flow exams analysis on 401 nasopharyngeal swab samples from non-duplicated suspected COVID-19 matters. An equal amount of UTM-containing samples (dilution ratio = 1:15) had been added to producer’s extraction buffer decision (dilution ratio = 1:2) and analyzed on BioSpeedia COVID19Tempo-Antigen Test and on Abbott PanbioTM COVID-19 Ag Speedy Test, items. Qualitative outcomes had been as compared with these obtained by the RT-qPCR (Allplex™ SARS-CoV-2 Assay Seegene).
Outcomes: Based on our information, normal sensitivity for BioSpeedia and Panbio items had been estimated at 65.5 and 75.0 %, respectively.
The sensitivity was higher for cycle threshold values decrease than 25 reaching 90.4 and 96.eight for BioSpeedia and Panbio items, respectively. A super specificity of 100.0% was seen for every items.
Conclusion: BioSpeedia and Panbio, as quick antigen exams with fantastic sensitivity to detect extreme viral a whole lot, appeared to be reliable choices to RT-qPCR notably useful when molecular testing functionality is also saturated by overflow of samples.
Given the extreme specificity of items, a optimistic final result would not require affirmation by a molecular examine and would as a consequence of this truth allow a sooner administration of COVID-19-positive victims. This textual content is protected by copyright. All rights reserved.
 Biotinylated Recombinant Human TGFR-1/ALK-5 Protein |
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| RP02598 | Abclonal | 500μg | EUR 422.5 |
) Human TGFR-1 / ALK-5 / TGF-beta receptor type-1 Protein, Llama IgG2b Fc Tag (SPR verified) |
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| TG1-H5259 | ACROBIOSYSTEMS | 100ug | EUR 2707.1 |
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Description: Human TGF-beta RI Protein, Llama IgG2b Fc Tag (TG1-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 34 - Leu 126 (Accession # P36897-1). |
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 Human TGFR ELISA Kit |
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| EHT0062 | Abclonal | 96Tests | EUR 625.2 |
 Active Recombinant Human TGFR-2 Protein |
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| RP00239 | Abclonal | 10 μg | EUR 35.75 |
 Rat TGFR ELISA Kit |
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| ERT0062 | Abclonal | 96Tests | EUR 625.2 |
 Goat TGFR ELISA Kit |
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| EGTT0062 | Abclonal | 96Tests | EUR 625.2 |
 Mouse TGFR ELISA Kit |
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| EMT0062 | Abclonal | 96Tests | EUR 625.2 |
 Sheep TGFR ELISA Kit |
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| EST0062 | Abclonal | 96Tests | EUR 625.2 |
 Monkey TGFR ELISA Kit |
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| EMKT0062 | Abclonal | 96Tests | EUR 625.2 |
 Rabbit TGFR ELISA Kit |
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| ERTT0062 | Abclonal | 96Tests | EUR 625.2 |
 Canine TGFR ELISA Kit |
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| ECT0062 | Abclonal | 96Tests | EUR 625.2 |
 Bovine TGFR ELISA Kit |
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| EBT0062 | Abclonal | 96Tests | EUR 625.2 |
 Porcine TGFR ELISA Kit |
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| EPT0062 | Abclonal | 96Tests | EUR 625.2 |
 Chicken TGFR ELISA Kit |
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| ECKT0062 | Abclonal | 96Tests | EUR 625.2 |
 Anserini TGFR ELISA Kit |
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| EAT0062 | Abclonal | 96Tests | EUR 625.2 |
 Guinea Pig TGFR ELISA Kit |
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| EGT0062 | Abclonal | 96Tests | EUR 625.2 |
 Human ALK-1 Antibody Pair Set |
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| ABPR-ZB169 | Creative Diagnostics | 5 plates, 15 plates | Ask for price |
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Description: Quantitative determination of Human ALK-1 |
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 Recombinant Human ALK-1 Protein |
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| VAng-2860Lsx-10g | Creative Biolabs | 10 µg | EUR 736.8 |
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Description: Recombinant Human ALK-1 was expressed in Sf9 Insect Cells. (Uniprot ID: P37023) |
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Recombinant Human ALK-1 Protein |
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| VAng-2860Lsx-1mg | Creative Biolabs | 1 mg | EUR 16605.6 |
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Description: Recombinant Human ALK-1 was expressed in Sf9 Insect Cells. (Uniprot ID: P37023) |
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 GOAT ANTI HUMAN IGG, INTACT IGG, ALK PHOS, 1 ML |
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| 9450431 | Lampire Biologicals Laboratories | 1ML | EUR 207.65 |
 RABBIT ANTI HUMAN IGG, INTACT IGG, ALK PHOS, 1 ML |
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| 9450511 | Lampire Biologicals Laboratories | 1ML | EUR 228.15 |
 Human ACVRL1 / ALK-1, Fc Tag |
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| E24PHA006 | EnoGene | 50 μg | EUR 180 |
 Human ACVRL1 / ALK-1, His Tag |
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| E24PHA007 | EnoGene | 50 μg | EUR 225 |
 Human ALK-1/ACVRL1 ELISA Kit |
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| EK5715 | SAB | 96 tests | EUR 599 |
) Goat anti Human IgG (Alk Phos) |
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| 43C-CB0963 | Fitzgerald | 1 mg | EUR 351 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43C-CB0978 | Fitzgerald | 1 mg | EUR 394 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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) Goat anti Human IgM (Alk Phos) |
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| 43C-CB0999 | Fitzgerald | 1 mg | EUR 347 |
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Description: Goat anti Human IgM secondary antibody (Alk Phos) |
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) Goat anti Human IgG (Alk Phos) |
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| 43R-1169 | Fitzgerald | 1 mg | EUR 167 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1175 | Fitzgerald | 1 mg | EUR 208 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-1192 | Fitzgerald | 1 mg | EUR 250 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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) Goat anti Human IgG (Alk Phos) |
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| 43R-1204 | Fitzgerald | 500 ug | EUR 293 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-IG012alk | Fitzgerald | 1 ml | EUR 522 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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Goat anti Human IgG (Alk Phos) |
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| 43R-IG165ALK | Fitzgerald | 1 mg | EUR 208 |
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Description: Goat anti Human IgG secondary antibody (Alk Phos) |
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) Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0949 | Fitzgerald | 1 mg | EUR 44 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0956 | Fitzgerald | 1 mg | EUR 387 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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Rabbit anti Human IgG (Alk Phos) |
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| 43C-CB0970 | Fitzgerald | 1 mg | EUR 387 |
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Description: Rabbit anti Human IgG secondary antibody (Alk Phos) |
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) Rabbit anti Human IgM (Alk Phos) |
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| 43C-CB9106 | Fitzgerald | 1 mg | EUR 425 |
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Description: Rabbit anti Human IgM secondary antibody (Alk Phos) |
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) Rabbit anti Human IgA (Alk Phos) |
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| 43C-CB9120 | Fitzgerald | 1 mg | EUR 385 |
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Description: Rabbit anti Human IgA secondary antibody (Alk Phos) |
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 Nori® Human ALK-1 ELISA Kit |
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| GR111542 | Genorise Scientific | 96-well | EUR 461 |
) Human ALK and LTK ligand 1 (ALKAL1) |
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| 1-CSB-EP757795HU | Cusabio |
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Description: Recombinant Human ALK and LTK ligand 1(ALKAL1) expressed in E.coli |
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) Anti-ACVRL1 / ALK-1 Reference Antibody (ascrinvacumab) |
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| E24CHA111 | EnoGene | 100 μg | EUR 225 |
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Description: Available in various conjugation types. |
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 GOAT ANTI HUMAN IGA, U CHAIN, INTACT IGG, ALK PHOS, 1 ML |
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| 9450381 | Lampire Biologicals Laboratories | 1ML | EUR 258.89 |
 GOAT ANTI HUMAN IGG, FCY FRAG, INTACT IGG, ALK PHOS, 1 ML |
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| 9450581 | Lampire Biologicals Laboratories | 1ML | EUR 258.89 |
 Human ALK-1 / ACVRL1 Protein, His Tag |
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| AL1-H5228 | ACROBIOSYSTEMS | 100ug | EUR 2140 |
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Description: Human ALK-1, His Tag (AL1-H5228) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Gln 118 (Accession # P37023). |
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 GOAT ANTI HUMAN IGM, FC5U FRAG, INTACT IGG, ALK PHOS, 1 ML |
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| 9450641 | Lampire Biologicals Laboratories | 1ML | EUR 269.14 |
 ALK-1 Polyclonal Antibody |
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| 41591-100ul | SAB | 100ul | EUR 302.4 |
ALK-1 Polyclonal Antibody |
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| 41591-50ul | SAB | 50ul | EUR 224.4 |
ALK-1 Polyclonal Antibody |
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| E041591 | EnoGene | 100μg/100μl | EUR 255 |
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Description: Available in various conjugation types. |
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ALK-1 Polyclonal Antibody |
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| E20-75182 | EnoGene | 100ug | EUR 225 |
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Description: Available in various conjugation types. |
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ALK-1 Polyclonal Antibody |
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| E20-70198 | EnoGene | 100ug | EUR 225 |
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Description: Available in various conjugation types. |
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ALK-1 Polyclonal Antibody |
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| ABP52913-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP52913-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP52913-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALK-1 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| ABP57032-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of ALK-1 from Human, Mouse, Rat. This ALK-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ALK-1 at AA range: 170-250 |
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ALK-1 Polyclonal Antibody |
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| E44H06624 | EnoGene | 100ul | EUR 255 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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ALK-1 Polyclonal Antibody |
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| E44H03961 | EnoGene | 100ul | EUR 255 |
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Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
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 ALK-1 Antibody, Concentrate |
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| MAB663C | Innovex | 0.2ml | EUR 475 |
 Rabbit Anti-Human ALK-1 monoclonal antibody, clone S215 |
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| CABT-ZB592 | Creative Diagnostics | 50 μl | EUR 520 |
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Description: Rabbit |
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 Rabbit Anti-Human ALK-1 monoclonal antibody, clone S135 |
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| CABT-ZB944 | Creative Diagnostics | 50 μl | EUR 520 |
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Description: Rabbit |
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 Human ALK-1/ACVRL1 PicoKine ELISA Kit |
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| EK1518 | BosterBio | 96 wells | EUR 510 |
|
Description: For quantitative detection of human ALK-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
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) OKRC00141-96W - ALK-1 ELISA Kit (Human) |
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| OKRC00141-96W | Aviva Systems Biology | 96Wells | EUR 550 |
) Goat anti Human IgG Fc (Alk Phos) |
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| 43-I24 | Fitzgerald | 100 ug | EUR 125 |
|
Description: Goat anti Human IgG Fc secondary antibody (Alk Phos) |
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 Recombinant Human ALK-1 Protein, His Tag |
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| E40KMP1394 | EnoGene | 20ug | EUR 495 |
 Anti-ALK-1 antibody |
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| STJ96547 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to ALK-1. |
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Anti-ALK-1 antibody |
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| STJ91563 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to ALK-1. |
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 Active Recombinant Human ALK-1/ACVRL1 Protein |
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| RP00978 | Abclonal | 5 μg | EUR 39 |
) Goat anti Human Kappa Chain (Alk Phos) |
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| 43C-CB9127 | Fitzgerald | 1 mg | EUR 403 |
|
Description: Goat anti Human Kappa Chain secondary antibody (Alk Phos) |
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) Goat anti Human kappa chain (Alk Phos) |
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| 43R-1257 | Fitzgerald | 500 ug | EUR 398 |
|
Description: Goat anti Human kappa chain secondary antibody (Alk Phos) |
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) Goat anti Human Lambda Chain (Alk Phos) |
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| 43C-CB9134 | Fitzgerald | 1 mg | EUR 403 |
|
Description: Goat anti Human Lambda Chain secondary antibody (Alk Phos) |
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 ALK-IN-1 |
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| T3059-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: ALK-IN-1 |
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ALK-IN-1 |
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| T3059-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: ALK-IN-1 |
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ALK-IN-1 |
|||
| T3059-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: ALK-IN-1 |
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ALK-IN-1 |
|||
| T3059-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: ALK-IN-1 |
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ALK-IN-1 |
|||
| T3059-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: ALK-IN-1 |
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ALK-IN-1 |
|||
| HY-13464 | MedChemExpress | 100mg | EUR 472.8 |
 ALK inhibitor 1 |
|||
| HY-15357 | MedChemExpress | 50mg | EUR 1712.4 |
ALK inhibitor 1 |
|||
| T10285-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: ALK inhibitor 1 |
|||
ALK inhibitor 1 |
|||
| T10285-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: ALK inhibitor 1 |
|||
ALK inhibitor 1 |
|||
| T10285-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: ALK inhibitor 1 |
|||
ALK inhibitor 1 |
|||
| T10285-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: ALK inhibitor 1 |
|||
ALK inhibitor 1 |
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| T10285-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: ALK inhibitor 1 |
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 (Alk Phos)) Goat anti Human IgG (H + L) (Alk Phos) |
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| 43C-CB0921 | Fitzgerald | 1 mg | EUR 152 |
|
Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
|||
| 43C-CB0928 | Fitzgerald | 1 mg | EUR 349 |
|
Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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Goat anti Human IgG (H + L) (Alk Phos) |
|||
| 43C-CB0935 | Fitzgerald | 1 mg | EUR 464 |
|
Description: Goat anti Human IgG (H + L) secondary antibody (Alk Phos) |
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