sphero ultra rainbow fluorescent particles
Description
Extremely Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with a number of fluorophores. They exhibit full spectrum fluorescence, together with enhanced UV and Far Crimson fluorescence. The combination of fluorophores permits Extremely Rainbow particles to be excited at any wavelength from 365 to 650nm. These particles can be found in two diameters, ~3.8µm and ~10.2µm.
Traits
Imply Diameter: ~3.8µm (3.61-3.99µm) or ~10.2µm (8.1 – 12.0µm);
see your Certificates of Evaluation for precise dimension.
Focus: ~2 x 106 particles/mL
Storage Buffer: Deionized water with 0.02% sodium azide and 0.01% NP-4O
Process
A dilution of 1-2 drops to 1mL will present an enough variety of particles for stream cytometry use.
Notes
Shake vigorously or vortex briefly earlier than use. Inclusion of a small quantity of detergent within the diluent will assist enhance the variety of singlets. Diluted particles may be saved within the fridge for future use.
Storage and Stability
Retailer at 2-8˚C. Freezing particles might lead to irreversible aggregation and lack of binding exercise. The product is anticipated to be steady for 12 months from the date of buy, offered that it’s dealt with in accordance with
the producer’s suggestions. The reagent needs to be saved in its opaque bottle.
Security
This particle suspension incorporates sodium azide. Sodium azide might react with lead and copper plumbing to type explosive metallic azides. Upon disposal of fabric, flush with a big quantity of water to forestall azide accumulation.
Please seek the advice of the Materials Security Information Sheet for extra info.
Description
This product incorporates a single inhabitants of Rainbow Particles which are dyed to a single fluorescent depth. Each Rainbow Particle incorporates a combination of fluorophores which are stably embedded inside the particle and may be excited at any wavelength from 365-650 nm, permitting most channels in a stream cytometer to be calibrated utilizing the identical set of particles. The Rainbow Particles have emission spectra appropriate with many frequent fluorophores used for immunofluorescent staining by stream cytometric evaluation. Rainbow Fluorescent Particles are offered in a dropper bottle for handy dishing out and storage.
| SPHERO Rainbow Fluorescent Particles – A single inhabitants of Rainbow Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels |
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Catalog No.
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Description
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Focus
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Nominal Dimension
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Bundle Dimension
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Value
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RFP-20-5
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Rainbow Fluorescent Particles (Depth just like brightest peak in RCP-20-5)
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107/mL
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1.8-2.2 µm
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5 mL
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$245.00
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RFP-30-5 (1 peak)
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Rainbow Fluorescent Particles (Depth just like brightest peak in RCP-30-5)
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107/mL
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3.0-3.5 µm
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5 mL
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$255.00
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RFP-30-5A (1 peak)
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Rainbow Fluorescent Particles (Depth just like mid vary FL1 fluorescence in RCP-30-5)
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107/mL
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3.0-3.Four µm
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5 mL
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$255.00
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RFP-35-5
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Rainbow Fluorescent Particles (Depth just like brightest peak in RCP-35-5)
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107/mL
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3.5-4.Zero µm
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5 mL
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$255.00
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RFP-50-5
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Rainbow Fluorescent Particles
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107/mL
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5.0-5.9 µm
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5 mL
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$265.00
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RFP-60-5
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Rainbow Fluorescent Particles (Depth just like brightest peak in RCP-60-5) (Beneficial for FL1, FL2, and FL3 channels solely)
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107/mL
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6.0-6.Four µm
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5 mL
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$265.00
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RFP-70-5
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Rainbow Fluorescent Particles
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107/mL
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6.5-8.Zero µm
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5 mL
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$265.00
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RFP-100-2
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Rainbow Fluorescent Particles
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107/mL
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8.1-12.Zero µm
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2 mL
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$210.00
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Beneficial Assay Procedures
This particle combination (~10×10^6 particles/mL) is beneficial for routine calibration of stream cytometers. Earlier than use, resuspend the particles by vortexing. Dilution of 3-5 drops of particles to 1 ml of sheath fluid will present an enough variety of particles for stream cytometric evaluation.
Product Notices
- Warning: Sodium azide yields extremely poisonous hydrazoic acid beneath acidic circumstances. Dilute azide compounds in working water earlier than discarding to keep away from accumulation of probably explosive deposits in plumbing.
- Cy is a trademark of GE Healthcare.
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