**Applied Biological Materials (abm) offers services and/or reagents specific to COVID-19 research. Submit a quote request containing the word ‘COVID-19’ to receive more details.**
Applied Biological Materials (abm) provides high quality research reagents and custom services at incredible prices! We have an extensive range of products and custom services to make abm your one-stop shop for all of your research needs. Our lineup includes:
Mycoplasma Decontamination & Detection Services.
Cell Immortalization and Stable Cell Line Generation.
Lentivirus Production.
Adenovirus Production.
AAV Production.
CHO Cell Antibody Production.
Illumina-Certified CSPro Next Generation Sequencing.
Making scientific discoveries shouldn’t cost you a fortune! Let abm be your primary research partner!
Please contact us for more information on any of our products and available services.
Description
Cryopreservation Medium by abm is a ready-to-use complete formulation suitable for the cryopreservation of various types of mammalian cells. With its unique formula this medium can be used to freeze cells cultured in classical media such as DMEM, DMEM/F12, RPMI 1640, and Ham’s F-12, as well as others.
This product is composed of 10% DMSO along with additional optimized components that will improve cell recovery after thawing. Every lot of Cryopreservation Medium by abm is tested for pH, sterility, and endotoxin.
Supplied by Applied Biological Materials (abm). Usually ships in 3 business days.
Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.
Mycoplasma Detection Kits – Conventional PCR
Mycoplasma contamination is a serious problem for laboratories due to its impact on cell properties and safety of biological products. Mycoplasma contaminations in cell cultures are unnoticeable (macroscopically as well as microscopically), making routine tests for reliable mycoplasma detection necessary. They represent a serious concern for academic and biopharmaceutical laboratories due to their effects on cell properties and on safety of biological products.
Mycoplasma (term often interchangeably used with Mollicutes, which include Mycoplasma, Acholeplasma, and Spiroplasma) are ubiquitous in the environment. In cell culture laboratories, 95 % of all mycoplasma infections in continuous cell lines are caused by as few as six contaminant species of bovine, swine, and human origin. These microbial agents easily spread from an infected cell culture batch to another by aerosol transmission or inadequate aseptic techniques.
Regular mycoplasma testing and control procedures help avoiding the costly consequences of a cell culture contamination and ensuring safe biopharmaceuticals and reliable scientific results. Consequently, most scientific publishers do not accept submission of cell-based studies without proof of the performed mycoplasma tests.
Compared to alternative methods, PCR-based mycoplasma detection kits are rapid, simple and do not require viable mycoplasma, special equipment, or extensive staff training.
Description: RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 17 bands, ranging from 50 bp – 1.5 kb, for molecular weight standards in agarose gel electrophoresis. The 200 and 500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Description: Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Description: Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 12 bands, ranging from 100 pb – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
The Mycoplasma PCR Detection Kit offers high specificity and sensitivity to minimize false positives while ensuring coverage over 200 strains of Mycoplasmas for quick and reliable routine screening of cell cultures less than 2 hours. Mycoplasmas are highly undesirable, easily acquired and notorious for an elusive onset of infection. It can alter the infected cells at a molecular level leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasmas in cell cultures is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. The MasterMix contains gel loading dye, making it convenient to load for gel electrophoresis.
Description: PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl.
Description: BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaq™ DNA polymerase, MgCl2, and other proprietary buffer components) have been developed for superb performance in sensitivity and signal-to-noise ratio. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments.
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments.
Description: BlasTaq™ DNA Polymerase is a strategically-engineered, next generation Taq Polymerasethat has rapid extension rates and robust performance. With specialized reactionconditions, this polymerase provides increased processivity, yields, and sensitivity, whileshortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonucleaseactivity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can beused with TA cloning vectors.
Description: abm’s qPCR Lentivirus Titer Kit is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and can be completed within 2 hours. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.