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Clinical performance of the Abbott Panbio with nasopharyngeal, throat, and saliva swabs among symptomatic individuals with COVID-19
SARS-CoV-2 antigen exams used on the point-of-care, such because the Abbott Panbio, have nice potential to assist fight the COVID-19 pandemic. The Panbio is Well being Canada accepted for the detection of SARS-CoV-2 in symptomatic people inside the first 7 days of COVID-19 symptom onset(s). Symptomatic adults just lately identified with COVID-19 in the neighborhood had been recruited into the examine. Paired nasopharyngeal (NP), throat, and saliva swabs had been collected, with one paired swab examined instantly with the Panbio, and the opposite transported in common transport media and examined utilizing real-time reverse-transcriptase polymerase chain response (RT-PCR). We additionally prospectively evaluated outcomes from evaluation facilities inside the group.
For these people, an NP swab was collected for Panbio testing and paired with RT-PCR outcomes from parallel NP or throat swabs. 100 and forty-five people had been included within the examine. Assortment of throat and saliva was stopped early as a result of poorer efficiency (throat sensitivity 57.7%, n=61, and saliva sensitivity 2.6%, n=41). NP swab sensitivity was 87.7% [n=145, 95% confidence interval (CI) 81.0-92.7%].
There have been 1641 symptomatic people examined by Panbio in evaluation facilities with 268/1641 (16.3%) constructive for SARS-CoV-2. There have been 37 false negatives and a couple of false positives, akin to a sensitivity and specificity of 86.1% [95% CI 81.3-90.0%] and 99.9% [95% CI 99.5-100.0%], respectively. The Panbio take a look at reliably detects most circumstances of SARS-CoV-2 from adults in the neighborhood setting presenting inside 7 days of symptom onset utilizing nasopharyngeal swabs. Throat and saliva swabs aren’t dependable specimens for the Panbio.
Analysis of the Panbio Leptospira IgM ELISA amongst Outpatients Attending Main Care in Southeast Asia
Regardless of estimates suggesting Leptospira spp. being endemic in Southeast Asia, proof stays restricted. Diagnostic accuracy evaluations based mostly on Leptospira ELISA primarily depend on hospitalized and extreme sufferers; subsequently, research measuring the pathogen burden could also be inaccurate in the neighborhood. We evaluated the Panbio Leptospira ELISA IgM amongst 656 febrile outpatients attending major care in Chiangrai, Thailand, and Hlaing Tha Yar, Yangon, Myanmar.
ELISA demonstrated restricted diagnostic accuracy for the detection of acute leptospiral an infection utilizing the producer beneficial cutoff, with a sensitivity of 71.4% and specificity of 36.4%, and an space below the receiver operator attribute curve worth of 0.65 (95% CI: 0.41-0.89), in contrast with our reference take a look at, the PCR assay. ELISA additionally carried out poorly as a screening device for detecting latest publicity to Leptospira spp. in contrast with the “gold-standard” microscopic agglutination take a look at, with a specificity of 42.7%. We conclude that the utility of the Leptospira IgM ELISA for each serodiagnosis and seroprevalence is restricted in our setting.
Nasopharyngeal Panbio COVID-19 Antigen Carried out at Level-of-Care Has a Excessive Sensitivity in Symptomatic and Asymptomatic Sufferers With Increased Threat for Transmission and Older Age
Background: Efficiency of point-of-care exams in several scientific eventualities and on completely different samples stays undetermined. We comprehensively evaluated the efficiency of the nasopharyngeal Panbio COVID-19 Ag Speedy Check Machine.
Strategies: It is a potential examine that features consecutive sufferers attending Three major care facilities (PCCs) and an emergency division. The antigen take a look at was carried out at point-of-care in nasopharyngeal and nasal swabs and in saliva. Constructive p.c settlement (PPA) and unfavourable p.c settlement (NPA) had been calculated with the reverse-transcription polymerase chain response (RT-PCR) assay as reference commonplace.
Outcomes: Of 913 sufferers included, 296 (32.3%) had been asymptomatic and 690 (75.6%) got here from the PCC. Nasopharyngeal swabs had been collected from 913 sufferers, nasal swabs had been collected from 659 sufferers, and saliva was collected from 611 sufferers. The RT-PCR was constructive in 196 (21.5%) nasopharyngeal samples (NPS). Total, PPA (95% CI) in NPS was 60.5% (53.3-67.4), and it was decrease in nasal swabs (44.7%) and saliva (23.1%). Check efficiency in NPS was largely depending on the cycle threshold (Ct) in RT-PCR, with PPA of 94% for Ct ≤25 and 80% for Ct <30.
In symptomatic sufferers, the PPA was 95% for Ct ≤25, 85% for Ct <30, and 89% for the symptom triad of fever, cough, and malaise. Efficiency was additionally depending on age, with a PPA of 100% in symptomatic sufferers >50 years with Ct <25. In asymptomatic sufferers, the PPA was 86% for Ct <25. In all circumstances, NPA was 100%.
Conclusions: The nasopharyngeal Panbio COVID-19 Ag take a look at carried out at point-of-care has an excellent sensitivity in symptomatic sufferers with Ct <30 and older age. The take a look at was helpful to determine asymptomatic sufferers with decrease Ct values.
Diagnostic efficiency of CerTest and Panbio antigen speedy diagnostic exams to diagnose SARS-CoV-2 an infection
Aims: Antigen speedy diagnostic exams (Ag-RDT) have been developed as dependable instruments to manage the SARS-CoV-2 pandemic. The target of our examine was to judge the diagnostic efficiency of two Ag-RDTs.
Strategies: We evaluated CerTest SARS-CoV-2 Ag One Step Card Check and Panbio COVID-19 Ag Speedy Check Machine Ag-RDTs. We included 320 nasopharyngeal samples: 150 PCR unfavourable samples to evaluate the specificity and 170 PCR constructive samples to judge the sensitivity. We additionally evaluated their sensitivity in keeping with cycle threshold (Ct) values and the time from the onset of signs. Assessments had been in contrast utilizing the McNemar’s take a look at and settlement was evaluated utilizing the kappa rating (okay).
Outcomes: Each Ag-RDTs confirmed a specificity of 100 %. Total sensitivity was 53.5 % for CerTest and 60.0 % for Panbio. For samples with Ct≤ 25, sensitivity was 94.0 % for CerTest and 96.4 % for Panbio (p = 0.500). Relating to samples with Ct>25, sensitivity was 14.0 % for CerTest and 24.4 % for Panbio (p = 0.004). Sensitivity for samples inside the first 5 days after the onset of signs had been 84.8 % for CerTest and 91.3 % for Panbio (p = 0.250) and notably decreased for samples taken after the fifth day. Each Ag-RDTs confirmed a superb settlement between them (settlement = 96.7 %, okay = 0.920). Settlement with PCR was additionally glorious for top viral load samples (Ct<25) for CerTest (98.0 %, okay = 0.954) and Panbio (98.8 %, okay = 0.973).
Conclusions: CerTest SARS-CoV-2 and Panbio COVID-19 Ag confirmed glorious efficiency and settlement outcomes for samples with excessive viral masses (Ct ≤ 25) or samples taken inside the first 5 days after the onset of signs.
Medical efficiency of the Panbio assay for the detection of SARS-CoV-2 IgM and IgG in COVID-19 sufferers
Following the SARS-CoV-2 pandemic, quite a few serological exams have been developed, together with speedy diagnostic exams. This examine goals at assessing the scientific efficiency of the Panbio IgG/IgM COVID-19 take a look at (Abbott), a speedy lateral movement assay for the qualitative detection of IgG and IgM in opposition to SARS-CoV-2. 100 and thirty-eight samples from 95 COVID-19 sufferers with a constructive SARS-CoV-2 RT-PCR had been analyzed to evaluate the scientific sensitivity. Seventy-six pre-COVID-19 samples had been used to judge the scientific specificity. Two impartial and blinded raters decided visually the presence or absence of the IgG, IgM and management traces for every take a look at after 10 and 20 minutes.
The sensitivity obtained with samples collected greater than 14 days after the onset of signs was 95.2% for IgG. IgM had been much less often detected (highest sensitivity of 20.5%). The specificities obtained had been 98.7% and 100% and for IgG and IgM respectively. As well as, the sensitivity of the assay was higher when the studying was carried out at 20 minutes than at 10 minutes, whereas the specificity was unchanged. The Panbio COVID-19 IgG/IgM speedy take a look at presents excessive sensitivities for IgG 14 days since symptom onset however low sensitivity for IgM. The specificity was glorious for each IgG and IgM. This text is protected by copyright.
 Alpha V Beta 5 Integrin, Peptide Aptamer, unlabeled |
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| AP-301-U | Alpha Diagnostics | 5 mg | Ask for price |
 Alpha V Beta 5 Integrin, Peptide Aptamer, Biotinylated |
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| AP-301-B | Alpha Diagnostics | 1 mg | Ask for price |
 Alpha V Beta 5 Integrin, Peptide Aptamer, FITC labelled |
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| AP-301-F | Alpha Diagnostics | 1 mg | Ask for price |
 Human Integrin alpha-X ELISA kit |
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| E01I0494-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Integrin alpha-X ELISA kit |
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| E01I0494-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Integrin alpha-X ELISA kit |
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| E01I0494-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Human Integrin alpha-X in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 anti- CD11c/Integrin alpha X antibody |
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| FNab01412 | FN Test | 100µg | EUR 606.3 |
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Description: Antibody raised against CD11c/Integrin alpha X |
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anti- CD11c/Integrin alpha X antibody |
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| FNab01413 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against CD11c/Integrin alpha X |
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 Anti-CD11c/Integrin alpha X antibody |
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| PAab01412 | Lifescience Market | 100 ug | EUR 426 |
 Integrin alpha X Antibody |
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| GWB-5111F7 | GenWay Biotech | 0.1 mg | Ask for price |
 Integrin alpha X antibody |
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| 70R-12673 | Fitzgerald | 100 ul | EUR 550 |
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Description: Affinity purified Rabbit polyclonal Integrin alpha X antibody |
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 Protein) Human Integrin Alpha X (ITGaX) Protein |
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| 20-abx166474 | Abbexa |
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 Rat Integrin alpha-X |
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| E01A14183 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Elisa Kit) Human Integrin, alpha X (CD11C) Elisa Kit |
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| EK713871 | AFG Bioscience LLC | 96 Wells | EUR 0.4 |
 ELISA Kit) Human Integrin Alpha X (CD11c) ELISA Kit |
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Human Integrin Alpha X (CD11c) ELISA Kit |
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| EKN53617-5x96T | Biomatik Corporation | 5x96T | EUR 2593.5 |
Human Integrin Alpha X (CD11c) ELISA Kit |
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| EKN53617-96T | Biomatik Corporation | 96T | EUR 546 |
Human Integrin Alpha X (CD11c) ELISA Kit |
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| EKU12505-48T | Biomatik Corporation | 48T | EUR 563.5 |
Human Integrin Alpha X (CD11c) ELISA Kit |
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| EKU12505-5x96T | Biomatik Corporation | 5x96T | EUR 3823.75 |
Human Integrin Alpha X (CD11c) ELISA Kit |
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| EKU12505-96T | Biomatik Corporation | 96T | EUR 805 |
 Human Integrin alpha- X, ITGAX ELISA KIT |
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| ELI-13625h | Lifescience Market | 96 Tests | EUR 988.8 |
 Goat Integrin alpha-X |
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| E01A49092 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 ELISA Kit) Human Integrin alpha-X (ITGAX) ELISA Kit |
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| RK11290 | Abclonal | 96T | EUR 280 |
 Polyclonal Antibody (Human)) Integrin Alpha X (ITGaX) Polyclonal Antibody (Human) |
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| 4-PAB159Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Integrin Alpha X (ITGaX) |
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 Sheep Integrin alpha-X |
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| E01A101385 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Mouse Integrin alpha-X |
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| E01A22925 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 CD11c/Integrin alpha X ELISA KIT|Human |
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| EF008489 | Lifescience Market | 96 Tests | EUR 826.8 |
 Canine Integrin alpha-X |
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| E01A66522 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Rabbit Integrin alpha-X |
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| E01A31659 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Monkey Integrin alpha-X |
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| E01A75239 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Bovine Integrin alpha-X |
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| E01A83957 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Human ITGAX / Integrin alpha-X ELISA Kit |
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| E9095h | EIAab | 96T | EUR 736 |
 Porcine Integrin alpha-X |
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| E01A57810 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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 Chicken Integrin alpha-X |
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| E01A92677 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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